Figure S2. Acute small molecule inhibitor treatments reveal stage-specific requirements of actomyosin contractility for proper heart development. Related to Figure 2I. (A) Schematic of different treatment periods and how they relate to embryonic development. Drawings after [S1]. (B) Stage 39 embryos exposed to acute stage-specific small molecule inhibitor treatments. Shape of heart drawn and expanded for emphasis (red). (C) The δcardiac morphology index is calculated from the morphology of a handdrawn region of interest (yellow dashed line; ROI) surrounding the larval heart (calculated values in Figure 2I). In addition to the heart (He) other features of the tadpole are visible including somites (So), cement gland (CG), and endoderm (En). Cardiac morphology (δcardiac morphology index) is calculated from the difference of the heart area from mean (δA), average control heart area (Ac), difference of circularity of the ROI from the mean (δCirc), and average control heart circularity (Circc). Scale bar = 500 μm.
Image published in: Jackson TR et al. (2017)
Copyright © 2017. Image reproduced with permission of the Publisher, Elsevier B. V.
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