Fig. 7. Repression and/or activation activities of Otx2-4E and -4A and Tle interaction. (A-D) WISH for gbx2 at the late gastrula to early neurula stage (stages 12.5-13). Dorsal view with anterior up. (A′,C′,D′) Enlarged images of A, C and D (dashed white box). (E-J) WISH for xcg1 at the late neurula stage (stages 18-20). Anterior view with dorsal up. CG indicates the cement gland. mRNA injection was the same as in Fig. 6. Colors of numbers and arrowheads are the same as in Fig. 4. (K-M) Luciferase reporter assays. meis3-D2-luc reporter DNA was co-injected with mRNA for Otx2-WT, -4E or -4A (50 pg/embryo) (K) or with combinations of mRNAs for Gsc (12.5 pg/embryo), Tle1 (12.5 pg/embryo), Otx2-WT and -4A (25 pg/embryo) as indicated (L). SOP-FLASH reporter DNA was co-injected with mRNA for Otx2-WT, -4E or -4A (50 pg/embryo) (M). **P<0.01 (Student’s t-test); error bars, s.e.m.; N.S., not significant; n indicates the number of samples. (N) Physical interaction between Tle1 and Otx2FL mutants as assayed by Co-IP. The amounts of expressed protein were verified by western blotting (lower panels). Apparent molecular masses (kDa) of Myc-Otx2FL-WT, -4A and -4E were 50, 50 and 57, respectively. Magenta arrowheads indicate co-immunoprecipitated bands; circles indicate heavy chains of IgG from the anti-Myc antibody. Scale bars: 300 μm in A-J,A′,C′,D′.
Image published in: Satou Y et al. (2018)
Copyright © 2018. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| gbx2.S | gbx2.1, XGBX-2 | X. laevis | Sometime during NF stage 12.5 to NF stage 13 | presumptive midbrain-hindbrain boundary chordal neural plate |
| muc2.L | cg-1, cg1, xcg, xcg-1, xcg1 | X. laevis | Sometime during NF stage 12.5 to NF stage 13 | cement gland primordium |
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