Network and molecular analysis of znf367 neighbors. (A) Distribution of membership values for the analyzed module in human cells and N. furzeri brain. The vertical line indicates the rank of znf367 and the horizontal line its membership value (black for N. furzeri and red for H. sapiens). Please note due to the high convexity of the human distribution ZNF367 has very high membership despite its rank. (B) Central part of the gene module containing ZNF367. Only genes showing topological overlap >0.3 are shown. ZNF367 is in red, while genes used for further analysis are in orange (SMC2, SKA3 and FANCD2). (C) qRT-PCR analysis of fancd2, ska3 and smc2. The levels of expression for the indicated mRNAs were evaluated for Co-MO or ZNF367-MO populations by qRT-PCR in triplicate and normalized to, glyceraldehyde 3-phosphate dehydrogenase (gapdh) expression. (D) mRNA distribution of fancd2, ska3 and smc2 in znf367 morphants. Neurula stage embryos are shown in frontal (D,E,F) and dorsal view (D’,E’,F’). Arrowheads indicated the expanded domain of the mRNA distribution of each gene in the injected side of the embryo. Abbreviations: n, number of independent experiments; Error bars indicate standard error of the means (s.e.m); n.s., not significant. *p ≤ 0,05; **p ≤ 0,01. P-value were calculated by Student’s T-test.
Image published in: Naef V et al. (2018)
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| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| fancd2.L | fa-d2, fa4, facd, fad, fad2, xfancd2 | X. laevis | Throughout NF stage 18 | neural plate pre-chordal neural plate chordal neural plate cranial neural crest migratory neural crest cell |
| ska3.L | rama1 | X. laevis | Throughout NF stage 18 | optic field neural plate pre-chordal neural plate chordal neural plate cranial neural crest migratory neural crest cell |
| smc2.L | xsmc2 | X. laevis | Throughout NF stage 18 | optic field neural plate pre-chordal neural plate chordal neural plate cranial neural crest migratory neural crest cell |
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