Fig. 7. The BCNE center is restricted to the dorsal-animal region by the maternally localized VegT transcription factor. (A) The phenotype of VegT-MO. White arrowhead indicates enlarged cement gland. (B) Control embryo for (A). (C) RT-PCR analyses of the effects of Wnt8 and VegT mRNA for animal caps. 1, 4, or 16 pg of Wnt8 mRNA and 100 pg of VegT mRNA were injected. (D) Chordin expression in control embryo at stage 9. White and black arrowheads indicate the animal and vegetal poles, respectively. (E) Siamois expression in control embryo. (F) Chordin expression of VegT-MO (16 ng) injected embryo. (G) Siamois expression of VegT-MO injected embryo. Note the expansion of Chordin and Siamois expression towards the vegetal side upon inhibition of VegT. (H) Real-time PCR analyses of Chordin mRNA expression levels from stage 8 to 12. (I) Siamois mRNA expression levels in the same samples. (J) Model of the regulation of the two blastula signaling centers. The border between the BCNE and Nieuwkoop centers is generated by repression by two maternally provided transcription factors: B1-type Sox (animal; Zhang et al., 2004) and VegT (vegetal).
Image published in: Ishibashi H et al. (2008)
Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V.
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