Fig. 5.slc7a5 depletion impaired neural tube closure and eye development.Loss-of-function experiment was performed with slc7a5 MO. (A, B) st.15 embryo. Anterior view. White arrowheads represent the neural fold. (A) Control MO-injectedembryo. (B) slc7a5 MO-injected embryo. The neural fold in the injected side was not distinct compared with that in the uninjected side. (C, D) st.20 embryo. Anteriorview. White arrowheads show the neural fold. (C) Control MO-injected embryo. (D) slc7a5 MO-injected embryo. The neural fold in the injected side did not fuse atthe midline and neural tube closure was not seen in the injected side. (E–X) st.35 embryo. (E–I) Control MO-injected embryo. (E) Uninjected side of control MO. (F)Injected side of control MO. Yellow broken line shows the plane of section in panel (G). (G) Transverse section of control MO-injected embryo. Two rectangles withred broken line represent regions magnified in panel (H) and (I). (H, I) Higher magnification of photomicrograph of rectangle (H) and (I) in panel (G). (J–S) Embryosinjected with slc7a5 MO. (J–N) Mild phenotype. (J) Uninjected side of slc7a5 MO-injected embryo. (K) Injected side of slc7a5 MO-injected embryo. Yellow brokenline shows the plane of section in panel (L). (L) Transverse section of slc7a5 MO-injected embryo with small eye. Two rectangles with red broken line representregions magnified in panel (M) and (N). (M, N) Higher magnification of photomicrograph of rectangle (M) and (N) in panel (L). (O–S) Severe phenotype. (O)Uninjected side of slc7a5 MO-injected embryo. (P) Injected side of slc7a5 MO-injected embryo. Yellow broken line shows the plane of section in panel (Q). (Q)Transverse section of slc7a5 MO-injected embryo with no apparent eye. Two rectangles with red broken line represent regions magnified in panel (R) and (S). (R, S)Higher magnification of photomicrograph of rectangle (R) and (S) in panel (Q). (T–X) Rescue by coinjection ofslc7a5.SmRNA andslc7a5.LmRNA with slc7a5 MO.(T) Uninjected side (i.e.no rescue). (U) Injected side of rescue constructs. Eye structure was rescued. Yellow broken line shows the plane of section in panel (V). (V)Transverse section of rescue constructs-injected embryo. Two rectangles with broken line represent regions magnified in panel (W) and (X). (W, X) Higher mag-nification of photomicrograph of rectangle (W) and (X) in panel (V). Arrow indicates the injected side. White brackets indicate the size of the eye.β-GalactosidasemRNA (1 ng) was used as a tracer (blue). Scale bars: 1 mm in A–D; 1 mm in E, F, J, K, O, P, T, U; 0.1 mm in G, L, Q, V; 0.1 mm in H, I, M, N, R, S, W, X. le: lens. (Forinterpretation of the references to color in thisfigure legend, the reader is referred to the web version of this article.)
Image published in: Katada T and Sakurai H (2019)
Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V.
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