Fig. 8.Inhibition of slc7a5 disturbed notochord forma-tion and diminished target genes in sonic hedgehog sig-naling.Notochordal markers and downstream molecules in sonichedgehog signaling were analyzed in slc7a5-depletedembryos at st.15. Arrow indicates the injected side. (A, B,E, F)chrd.1expression. (A, B) Dorsal view. (E, F)Transverse section. (A) Control MO-injected embryo. (B)slc7a5 MO-injected embryo. Expression ofchrd.1was notextended enough along the anteroposterior axis and dif-fused laterally in slc7a5 MO-injected side (white arrow-heads). (E) Transverse section of control MO-injectedembryo. (F) Transverse section of slc7a5 MO-injectedembryo. Expression ofchrd.1diffused laterally (whitearrowheads). (C, D, G, H)shhexpression. (C, D) Dorsalview. (G, H) Transverse section. (C) Control MO-injectedembryo. (D) slc7a5 MO-injected embryo. Faint expressionofshhwas observed similar tochrd.1expression (whitearrowheads). (G) Transverse section of control MO-in-jected embryo. (H) Transverse section of slc7a5 MO-in-jected embryo. Expression ofshhdiffused laterally similartochrd.1expression (white arrowheads). (I, J, M, N)ptch2expression. (I, J) Dorsal view. (M, N) Transversesection. (I) Control MO-injected embryo. (J) slc7a5 MO-injected embryo.ptch2was vanished in the injected side(yellow arrowheads). (M) Transverse section of controlMO-injected embryo. (N) Transverse section of slc7a5MO-injected embryo. Expression ofptch2was diminishedin the injected side (yellow arrowhead). (K, L, O, P)foxa2(HNF-3β) expression. (K, L) Dorsal view. (O, P)Transverse section. (K) Control MO-injected embryo. (L)slc7a5 MO-injected embryo. Expression offoxa2wasweakened in the injected side (white arrowheads). (O)Transverse section of control MO-injected embryo. (P)Transverse section of slc7a5 MO-injected embryo.Expression offoxa2was diffused in the injected side(white arrowhead). (Q, R)gli1expression. Anterior view.slc7a5 depletion impairedgli1expression (yellow ar-rowheads). (S, T)gli2expression. Anterior view.Expression ofgli2was abolished in the slc7a5 MO-in-jected side similar to that ofgli1(yellow arrowheads).β-GalactosidaseRNA (1 ng) was used as a tracer. (U) RT-qPCR was performed with slc7a5-depleted embryos.Expression level of these genes was down-regulatedespecially in shh signaling family genes. Scale bars: 1 mmin A–D, I–L, Q–T; 0.1 mm in E–H, M–P. (For interpreta-tion of the references to color in thisfigure legend, thereader is referred to the web version of this article.)
Image published in: Katada T and Sakurai H (2019)
Copyright © 2019. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| chrd.L | chd, chordin, chrd.1, LOC108716939, X-chordin | X. laevis | Throughout NF stage 15 | notochord |
| ptch2.L | patched 2, patched-2, ptc-2, ptc2, ptch2-a, ptch2-b, xptc 2, xptc-2 | X. laevis | Throughout NF stage 15 | neural plate notoplate |
| gli1.L | Gli-1, X-gli1 | X. laevis | Throughout NF stage 15 | neural plate neural fold |
| shh.L | hedgehog, vhh-1, X-SHH, Xhh, xshh | X. laevis | Throughout NF stage 15 | neural plate notochord |
| foxa2.L | HNF-3B, hnf3-beta, hnf3b, hnf3beta, LOC108717763 | X. laevis | Throughout NF stage 15 | notoplate neural plate |
| gli2.L | gli4 | X. laevis | Throughout NF stage 15 | neural fold neural plate |
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