Fig. S2. Expression profiles of two housekeeping genes, prps1 and dicer1. Expression profiles of two housekeeping genes, prps1 and dicer1, by RNA-seq (A) (Owens et al., 2016) and by qPCR (B) are shown. ee, mature RNA. ei, precursor RNA. The RNA-seq data was retrieved from http://genomics.crick.ac.uk/apps/profiles/. qPCR for all primer sets were performed with RT- samples (represented in black) to detect traces of contaminated genomic DNA. The copy numbers of pre-spliced prps1 (ei) (magenta) were also low, but reached to about 20000 transcripts per reaction at 18.5 hpf. The amount of total RNA extracted per embryo was not different among stages, and the same amount of RNA was used per qPCR reaction. Therefore, the expression profiles of prps1 and dicer1 from RNA-seq and qPCR (ee) are directly comparable.
Image published in: Kondo M et al. (2019)
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