Fig. 5. The Tbx2 C-terminus is required for mesendoderm suppression. A: Expression of Tbx2DC does not suppress mesoderm induction by Activin. RT-PCR analysis of animal caps dissected at late blastula stages and cultured until mid-gastrula stages. tbx2DC RNA (1 ng) was injected at early cleavage stages, as indicated. Activin (0.5 ng/ml) was added to stage 8 animal caps, as indicated. B: Expression of Tbx2DC stimulates expression of mesodermal marker genes in the absence of exogenous growth factors. RT-PCR analysis of animal caps dissected at late blastula stages and cultured until mid-gastrula stages. tbx2DC (1 ng) was injected at early cleavage stages, as indicated.
Image published in: Teegala S et al. (2018)
Copyright © 2018. Image reproduced with permission of the Publisher, John Wiley & Sons.
Permanent Image Page
Printer Friendly View
XB-IMG-174931