FIGURE 2 KillerRed‐induced hypertrophy is caused by superoxide production. (a) (i) Exposure of KillerRed (KR) expressing tadpoles to green light at NF stage 42 produces superoxide radicals, indicated by an increase in dihydroethidium (DHE) fluorescence in the heart (outlined). “L” marks the liver, which is autofluorescent. (ii) Without light activation, KillerRed produces no superoxides. (iii) Exposure of KillerRed‐expressing tadpoles to the ROS scavenger edaravone (EDV) prevents light‐induced production of superoxides. All scale bars are 100μm. (b) Exposure of KillerRed‐expressing tadpole hearts to EDV suppresses the light‐induced ventricular hypertrophy seen in DMSO controls. Mean ventricular volume is shown (n = 34–39 tadpoles, N = 2 replicates), with error bars indicating 95% confidence intervals. *p < 0.05; **p < 0.01 (ANOVA with Tukey post hoc)
Image published in: Jewhurst K and McLaughlin KA (2019)
Copyright © 2019. Image reproduced with permission of the Publisher, John Wiley & Sons.
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