Fig.m 9. Global activation of adhesion in vivo is sufficient to rescue Cxcr4 loss-of-function. a, b Fibronectin immunostaining on cryosections through the cephalic region of St17 (a) and St20 (b) Xenopus laevis embryos. c Diagram depicting the grafting procedure. d Representative images of the three types of grafts that were performed. Controls NC cells (FDx), cells injected with Cxcr4MO with or without prior exposure to Mn2+ were grafted into control non-injected hosts embryos. e Normalised net distance of migration along the dorso-ventral axis of grafted cells after an overnight incubation following the graft, n = 43 grafted embryos from four independent experiments. ANOVA followed by multiple comparisons, p values are indicated on the graph. f Still images from time-lapse movies for Cxcr4MO cells co-injected with CIBN-GFP grafted into control embryos. g Still images from time-lapse movies for Cxcr4MO cells co-injected with CIBN-GFP and photoactivatable Tiam1 grafted into control embryos. Arrows indicate NC cells migrating along the dorso-ventral axis. h Normalised net distance of migration along the dorso-ventral axis of grafted cells after 6 h of time-lapse imaging, n = 33 grafted embryos from five independent experiments. Student’s t-test, two tailed, p value indicated on the graph. FDx, fluorescein dextran; NC, neural crest; No, notochord. Scale bar in a, 50 μ. Scale bars in d and f, 500 μ. Box and whiskers plot: the box extends from the 25th to the 75th percentile; the whiskers show the extent of the whole dataset. The median is plotted as a line inside the box. Source data are provided as a Source Data file
Image published in: Bajanca F et al. (2019)
© The Author(s) 2019. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
Permanent Image Page
Printer Friendly View
XB-IMG-175707