Figure 4. Wnt5a induces redistribution of Ror2 homodimers and Ror2/dsh heterodimers. The subcellular localization of fluorophore tagged Ror2 homodimers (a) and Ror2/dsh heterodimers (b) was analyzed by a bimolecular fluorescence complementation assay (BiFC) in transiently transfected Xenopus tissue culture cells. The relative grey values show the intensity profiles at the positions marked by yellow bars. (c,d) The membrane/cytoplasm values were calculated in the following way: membrane/cytoplasm = (intensity at the membrane – intensity outside)/(intensity inside – intensity outside). Shown are the mean values and standard errors of at least 399 profiles of more than 60 cells from at least ten independent experiments. Median and dispersion (25–75% range) are shown as box-and-whiskers plots in Supplementary Fig. 5b. (d) The effect of endocytosis inhibitors chlorpromazine and genistein on the subcellular distribution of Ror2/dsh heterodimers. Shown are the mean values and standard errors of at least 280 profiles of more than 40 cells in at least seven independent experiments. p values according to Student’s t-test, n.s.: not significant. Median and dispersion are shown in Supplementary Fig. 5c.
Image published in: Puzik K et al. (2019)
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