Figure 6. Caveolin regulates internalization of Wnt5a/Ror2 clusters. (a) Colocalization of Ror2mCherry/Wnt5aeGFP clusters in animal cap explants of caveolin1 and caveolin3 morphants and caveolin1 and caveolin3 overexpressing embryos. (b,c) Quantification of the clusters. More than 80% of the clusters are located at the plasma membrane. Overexpression of caveolin1 slightly increases the frequency of membrane localization. (c) Average number of intracellular clusters per cell. p values according to Students t-test. (d) The BiFC signal of Ror2/dsh dimers partially colocalizes with the red fluorescence of caveolin1mGarnet in transfected XTC-cells, both, at the plasma membrane and in the cytoplasm (arrowheads in the close-ups). (e) Single particle tracking using widefield fluorescence microscopy indicates that cav1eGFP (blue trajectory) and Ror2mcherry (red trajectory) co-migrate in the cytoplasm (arrowheads) and leave the membrane together and continue co-migrating in the cytoplasm (arrows).
Image published in: Puzik K et al. (2019)
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