Fig. 6. Expression of myc-ID dimer derepresses T3-response genes in premetamorphic tadpoles. Wild type (WT) and myc-ID dimer transgenic (Tg) tadpoles at stage 46 were heat shocked for 1 h per day. Total RNA was isolated from individual animals before (day 0) and after 1 and 5 days of heat shock treatment. Each group had four individuals. The cDNAs reverse-transcribed from the total RNAs were subjected to qPCR analysis as in Fig. 5. White bars represent expression levels in WT animals and black bars represent those in Tg tadpoles. Note that expression of T3-response genes (TRβ, ST3, TH/bZip, and xhh) in Tg animals tended to be higher than WT counterparts at most of time points (∗p < 0.05), whereas such tendency was not seen in HoxA1 (slightly lower at one time point), a retinoic acid-response gene. Similar results were obtained from another experiment with an independent batch of animals.
Image published in: Sato Y et al. (2007)
Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V.
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