Fig. 1. Blocking retinoic acid signaling at stage 12/14 results in a failure to form a simple heart tube. Whole-mount in situ hybridization for cTnI was used to visualize the differentiated myocardium (blue staining). In stage 31 embryos, a normal linear heart tube that is just starting to loop (A). The tube and looping are best viewed from the ventral side of the embryo (B). When embryos are treated with 1 μM RA, the heart is severely disrupted (C), although when viewed from the ventral side, it can be seen that a tube is still formed (D). When treated with 1 μM AGN 194301, myocardial differentiation still occurs (E), but when viewed from the ventral side, the lack of tube formation is clear (F). Addition of equimolar (1 μM) concentrations of both RA and AGN 194301 resulted in an apparently normal heart tube indicating that the observed effects are not due to toxicity of the compounds used (G, H).
Image published in: Collop AH et al. (2006)
Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V.
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