Fig. 5. Combinatorial activity of FGF and wnt signaling. Panel A shows the phenotype at swimming larva stage 41 of control embryos and embryos injected with 2 ng FrzB mRNA or 2 ng dnFGFR1 mRNA or 2 ng FrzB mRNA + 2 ng dnFGFR1 mRNA. Panel B shows whole-mount in situ hybridization analysis for Cdx1, Cdx2 and Cdx4 expression at gastrula stage 11 in control embryos and embryos injected with 2 ng FrzB mRNA or 2 ng FrzB mRNA + 2 ng dnFGFR1 mRNA. Panel C is an RPA showing the expression of Cdx genes at gastrula stage 11 in control embryos and embryos injected with 2 ng dnFGFR1 mRNA or 2 ng FrzB mRNA or 2 ng FrzB mRNA + 2 ng dnFGFR1 mRNA. Panel D is an RPA showing Cdx gene expression at control stage 13 in control animal cap explants and animal cap explants from embryos injected with 10 pg FGF4 mRNA or 50 pg wnt8 mRNA or 10 pg FGF4 mRNA + 50 pg wnt8 mRNA. Panel E is a bar chart showing the relative levels of expression as determined by densitometric analysis of each Cdx gene in the animal caps analyzed in panel D. Expression levels are normalized to the ODC loading control and the expression of each gene is shown relative to expression in untreated control caps. Panel F is a Western blot of control embryos and embryos injected with 2 ng dnFGFR1 mRNA or 2 ng FrzB mRNA or 2 ng FrzB mRNA + 2 ng dnFGFR1 mRNA probed for p-S473 Akt, dp-ERK and GAPDH at gastrula stage 10.5. Panel G shows whole-mount in situ hybridization analysis of wnt8 expression at gastrula stage 11 in control embryos and embryos injected with 4 ng dnFGFR1 mRNA.
Image published in: Keenan ID et al. (2006)
Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| cdx1.S | cdx-1 | X. laevis | Throughout NF stage 11 | marginal zone circumblastoporal collar dorsal marginal zone |
| cdx2.L | cad2, cdx-2, cdx3-like, xcad2, Xcaudal2 | X. laevis | Throughout NF stage 11 | marginal zone dorsal marginal zone circumblastoporal collar |
| cdx4.L | cad3, MGC130769, MGC68909, Xcad-3, Xcad3, Xtcad3 | X. laevis | Throughout NF stage 11 | marginal zone dorsal marginal zone circumblastoporal collar |
| wnt8a.L | wnt-8, wnt8, Xwnt-8, Xwnt8 | X. laevis | Throughout NF stage 11 | mesoderm marginal zone |
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