Fig. 7. (A) Xmxi1 induces neuronal differentiation in neuralized late, but not early animal caps. Animal caps were harvested from embryos injected with noggin (50 pg) and MT-Xmxi1 (500 pg), as indicated. At the equivalent of stage 14 or 20, the caps were harvested and analyzed by real-time RT-PCR. Expression levels were normalized to ODC and compared to control caps, which were set to 1. (B–E) The inhibition of neuronal differentiation by Xmxi1 is only transient. At tailbud stages, MT-Xmxi1 (750 pg) ectopic Sox3 is still present (B) and ectopic N-tubulin expression is also detected (D). (C, E) Transversal sections of B and D, respectively. Red arrowhead marks ectopic expression.
Image published in: Klisch TJ et al. (2006)
Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| sox3 | Sry, Xlsox3, xSox3, Xtsox3 | Xenopus | Throughout NF stage 24 | brain midbrain hindbrain spinal cord |
| tubb2b.S | N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin | X. laevis | Throughout NF stage 24 | brain midbrain hindbrain spinal cord |
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