Figure 4. Suppression of endogenous expression of Xbra by XSUMO-1-MO. A-F: Embryos were coinjected with indicated RNA or MO and lacZ (lineage tracer) into the dorsal marginal zone at the 8-cell stage. No injection: Nothing (A), MO + mRNA: coinjected with 40 ng XSUMO-1-MO and 1 ng myc-XSUMO-1 mRNA (D and F), and 40 ng Control MO or XSUMO-1-MO (B, C, and E) was injected. Red and blue indicate the injected area and Xbra-expressing area, respectively. Xbra expression was inhibited in the XSUMO-1-MO-injected area (C and E, arrow). This inhibition was rescued by coinjection with myc-XSUMO-1 mRNA (D and F). A-D: Dorsal is up, vegetal pole is front. E and F: Animal pole is up, dorsal is front. All embryos shown were at the early gastrula stage (stage 11).
Image published in: Yukita A et al. (2007)
Copyright © 2007. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| tbxt.S | bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury | X. laevis | Throughout NF stage 11 | marginal zone |
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