Fig. 4. Evi1 expression in the pronephros is upregulated by retinoid signaling and repressed by xWT1 and Notch signaling. Embryos were injected with the indicated mRNA together with β-galactosidase mRNA and processed at tailbud stage by whole-mount in situ hybridization to visualize Evi1 expression. In all panels, anterior is to the right. Control and injected sides revealed with X-gal are shown. (A–H) Evi1 expression is elevated in embryos by RA treatment or by injection of mRNA encoding XRALDH2 in the presence of its substrate, all-trans retinal. Conversely, it is decreased in embryos where RA signaling is inhibited by injection of xCYP26 mRNA or the dominant negative RARα receptor xRARα1405*. (I–L) Evi1 expression is increased in embryos where Notch signaling is inhibited by injection of mRNA encoding the dominant negative form of Su(H), Su(H)DBM. Conversely, a reduction of Evi1 expression is observed in embryos overexpressing the activated form of Notch, NICD. (M, N) Evi1 expression is downregulated by xWT1 mRNA injection.
Image published in: Van Campenhout C et al. (2006)
Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| mecom.L | ecotropic viral integration site 1, evi-1, evi1, mecom-a, mecom-b, xEvi-1 | X. laevis | Sometime during NF stage 20 to NF stage 22 | somatic layer of lateral plate mesoderm |
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