Fig. 1. Maternal and early zygotic expression of dnah9. Whole-mount in situ hybridization of staged embryos with a dnah9-specific antisense probe. (A, B) Maternal mRNA localizes to the animal pole of the zygote (hemi section in A), and 4-cell embryo in top (B) and side view (B’). (C–F) Zygotic expression in the dorsal lip at stage 11 (C) and at stage 13 (arrow in D). (E) Early neurula (stage 16) in frontal view (E) and transverse section (E’). Note expression in the floor plate (arrowheads; section at a level anterior to the gastrocoel roof plate). (F) Lateral view of a stage 21 neurula with floor plate (F’) and epidermal staining (anterior to the left). (G) Localization of dnah9 mRNA in multi-ciliated cells of the epidermis at stage 32, demonstrated by sequential in situ hybridization (G’) and immunohistochemistry using an antibody against acetylated tubulin (G”). no, notochord; s, somite. Scale bar represents 15 μm.
Image published in: Vick P et al. (2009)
Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| dnah9.S | dnah9-A, L-R Dynein, Left-Right Dynein, LRD | X. laevis | Throughout NF stage 1 to NF stage 3 (4-cell) | animal hemisphere |
| dnah9.S | dnah9-A, L-R Dynein, Left-Right Dynein, LRD | X. laevis | Throughout NF stage 11 | dorsal marginal zone |
| dnah9.S | dnah9-A, L-R Dynein, Left-Right Dynein, LRD | X. laevis | Throughout NF stage 16 | floor plate neural groove neuroectoderm pre-chordal neural plate |
| dnah9.S | dnah9-A, L-R Dynein, Left-Right Dynein, LRD | X. laevis | Throughout NF stage 21 | floor plate epidermis ectoderm neuroectoderm pre-chordal neural plate |
| dnah9.S | dnah9-A, L-R Dynein, Left-Right Dynein, LRD | X. laevis | Throughout NF stage 32 | ciliated epidermal cell epidermis ectoderm neuroectoderm ectodermal cell |
| dnah9.S | dnah9-A, L-R Dynein, Left-Right Dynein, LRD | X. laevis | Throughout NF stage 13 | upper blastopore lip ectoderm neuroectoderm |
| tuba4b.L | alpha tubulin, alpha-tubulin, tuba4, tuba4a | X. laevis | Throughout NF stage 32 | ciliated epidermal cell epidermis ectoderm |
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