Fig. 1. Maternal and early zygotic expression of dnah9. Whole-mount in situ hybridization of staged embryos with a dnah9-specific antisense probe. (A, B) Maternal mRNA localizes to the animal pole of the zygote (hemi section in A), and 4-cell embryo in top (B) and side view (B’). (C–F) Zygotic expression in the dorsal lip at stage 11 (C) and at stage 13 (arrow in D). (E) Early neurula (stage 16) in frontal view (E) and transverse section (E’). Note expression in the floor plate (arrowheads; section at a level anterior to the gastrocoel roof plate). (F) Lateral view of a stage 21 neurula with floor plate (F’) and epidermal staining (anterior to the left). (G) Localization of dnah9 mRNA in multi-ciliated cells of the epidermis at stage 32, demonstrated by sequential in situ hybridization (G’) and immunohistochemistry using an antibody against acetylated tubulin (G”). no, notochord; s, somite. Scale bar represents 15 μm.
Image published in: Vick P et al. (2009)
Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V.
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