Fig. 5. No leftward flow at the GRP of dnah9-SB and dnah5-SB morphant embryos. Flow was analyzed by addition of fluorescent microbeads to dorsal explants and video microscopy. Representative examples of stage 17/18 dorsal explants of Co-MO (A), dnah9-SB-MO (B) and dnah5-SB-MO (C) injected embryos. Targeted areas indicated by red lines represent the limits of lineage tracer. Particle movements displayed as gradient-time trails (GTTs), representing 25 s from green to red (cf. bar in C). (A’–C’) Quantitative analysis of GTT directionality over the area of the GRP demonstrating strong leftward flow in Co-MO and absence of directed bead transport in dnah9-SB- and dnah5-SB-MO-injected specimen. (D, E) Collapsed movies of in vivo imaged cilia movements by fluorescence microscopy using a PACRGeGFP fusion construct. Co-MO injection resulted in wildtype rotational pattern (D), whereas dnah9-SB-MO-injected GRPs displayed variant phenotypes, namely irregular circular movements (E), wiggling (E’) or arrested motion (E”; cf. Suppl. Movie 5). Scale bar represents 50 μm. a, anterior; l, left; p, posterior; r, right.
Image published in: Vick P et al. (2009)
Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V.
| Experiment + Assay | Source | Phenotypes and Disease | ||
|---|---|---|---|---|
| Xla Wt + dnah5 MO + NF31 (whole-mount microscopy) | fig.5.b |
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