Fig. 8. Sox2 inhibits canonical Wnt/β-catenin signaling. (A,B) mRFP-only-injected cells co-express the TOP-dGFP reporter (A, arrowheads), unlike Sox2-injected cells (B). (C) The proportion of TOP-dGFP+ cells out of the total injected (RFP+) cells is reduced in the presence of Sox2 (mRFP, n=13, 3471 cells; Sox2, n=10, 3420 cells). (D) mRNAs for mRFP (tracer) alone, mRFP+Sox2 or mRFP+Sox2BD(-) (dominant-negative Sox2) were injected into dorsal animal cells of cleavage-stage embryos. TOP-dGFP DNA was subsequently lipofected into stage 17 optic vesicles. (E-G) In a Sox2-injected embryo, the cells that express GFP (E) are those that do not express Sox2 (gaps indicated by arrowheads in F). (H) In retinal cells expressing Sox2 or Sox2BD(-), there is a 2-fold reduction in the proportion of TOP-dGFP+ cells that express mRFP (mRFP, 682 cells; Sox2, 228 cells; Sox2BD(-), 437 cells). Error bars indicate 95% C.I. *P<0.01.
Image published in: Agathocleous M et al. (2009)
Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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