Fig. 3. Effect of the inhibition of PI3K signalling on the in vivo expression of early patterning genes. (A-K) Embryos were injected equatorially at the four-cell stage in one or two blastomeres with mRNA for NLS-b-Gal alone (A,D,F,H,J), with ã p85 (0.5 ng/blastomere; B,E,G,I,K), or with ã p85 (0.5 ng/blastomere) and p110caax (0.5 ng/blastomere, C), and cultured until stage 10.5-11 before fixing and whole-mount in situ hybridisation with the indicated probe (top right of each part). (L) RT-PCR analysis of the expression of early trunk mesodermal markers in stage 10.5 whole embryos (WE) treated between stage 8.5 and 10.5 with 100 mM of LY294002 (LY). (M) RT-PCR analysis of the expression of early mesodermal and endodermal markers in stage 10.5 whole embryos (WE) treated between stage 8.5 and 10.5 with 100 mM of LY294002 (LY). (N) RT-PCR analysis of the expression of Xbra and Siamois (Sia) at stage 10 in control embryos or embryos incubated in LY294002 (LY; 25 mM) between the four-cell stage and stage 10.
Image published in: Carballada R et al. (2001)
Copyright © 2001. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| tbxt.S | bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury | X. laevis | Sometime during NF stage 10.5 to NF stage 11 | marginal zone |
| chrd.S | chd, chordin, chrd.1, LOC108716939, X-chordin | X. laevis | Sometime during NF stage 10.5 to NF stage 11 | dorsal marginal zone |
| gsc.L | goosecoid, gsc-a, gsc-b, Xgsc | X. laevis | Sometime during NF stage 10.5 to NF stage 11 | dorsal marginal zone |
| mixer.L | tMixer | X. laevis | Sometime during NF stage 10.5 to NF stage 11 | endoderm |
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