Fig. 3. In situ hybridization of XrGAP. (A) Laternal view of a blastula (stage 8) embryo showing enhanced signal in the animal hemisphere. (B) Vegetal view of a stage 10 embryo. (C) Lateral view of a sagitally sectioned stage 10.5 embryo. XrGAP transcripts are detected in the prospective neuroectoderm and the mesoderm with more enrichment at the dorsal marginal zone. An arrowhead indicates dorsal lip of blastopore. XrGAP expression is also detected in the dorsal involuting cells (arrow). Anterior view (D) and dorsal view (E) of mid-neurula (stage 16) embryo. XrGAP is expressed at the neural folds from anterior to caudal end, but absent from the dorsal midline. Note the expression in eye anlange (two arrowheads) and neural folds (two arrows). (F) Anterior view of late neurula (stage 20) embryo. XrGAP expression remains restricted to eye anlagen (two arrowheads) and antero-ventral portions of neural regions as a crescent-shape. (G) Lateral view of a sagittally sectioned stage 23 embryo. Expression is observed in notochord but absent from other mesodermal tissues. (H) Anterior view of a stage 25 embryo. XrGAP expression remains restricted in the developing brain and eye vesicle (two arrowheads). (I) Lateral view of a tailbud (stage 30) embryo. XrGAP expression persists in the developing brain, spinal cord, eye, branchial arches, and otic vesicle. ba; branchial arches, cg, cement gland; nc, notochord; ey, eye; ov, otic vesicle.
Image published in: Kim J et al. (2003)
Copyright © 2003. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| arhgap21.L | Rho-GTPase activating protein 10, xrgap | X. laevis | Throughout NF stage 10.5 | mesoderm neuroectoderm inner layer neuroectoderm outer layer dorsal marginal zone blastopore lip |
| arhgap21.L | Rho-GTPase activating protein 10, xrgap | X. laevis | Throughout NF stage 16 | anterior neural fold pre-chordal neural plate neuroectoderm neural plate |
| arhgap21.L | Rho-GTPase activating protein 10, xrgap | X. laevis | Throughout NF stage 20 | eye primordium neuroectoderm anterior neural fold |
| arhgap21.L | Rho-GTPase activating protein 10, xrgap | X. laevis | Throughout NF stage 23 | notochord brain eye primordium anterior neural tube |
| arhgap21.L | Rho-GTPase activating protein 10, xrgap | X. laevis | Throughout NF stage 25 | brain optic vesicle spinal cord neural crest eye head otic placode |
| arhgap21.L | Rho-GTPase activating protein 10, xrgap | X. laevis | Sometime during NF stage 28 to NF stage 29 and 30 | spinal cord branchial arch otic vesicle brain eye lens placode cranial placode |
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