Fig. 3. Inhibition of Smicl function causes down regulation of Chordin expression, but not of other mesodermal and endodermal markers. (A-E) Expression levels, normalised to that of ornithine decarboxylase, of Xbra (A), Sox17 (B), Xnr3 (C), Goosecoid (D) and Chordin (E) in uninjected embryos, embryos injected with morpholino oligonucleotide coMO (80 ng), or embryos injected with morpholino oligonucleotide XlMO (80 ng). RNA was extracted at the indicated stages, and RNA levels were analysed by quantitative RT-PCR. (F,G) Inhibition of Smicl function by means of morpholino oligonucleotide XtMO1 (5 ng; F) or XtMO2 (30 ng; G) also causes downregulation of Chordin expression in Xenopus tropicalis. Embryos were assayed at stages 9, 9.5, 10 and 10.5 (F), or at stage 10.5 (G). (H) Injection of RNA encoding mouse Smicl causes the upregulation of Chordin in embryos injected with morpholino oligonucleotide coMO and rescues the downregulation of Chordin caused by morpholino oligonucleotide XlMO. Embryos were injected with the indicated RNAs (1 ng) or morpholino oligonucleotides (50 ng). RNA was extracted at stage 10.5 and expression of Chordin and ornithine decarboxylase was assayed by quantitative RT-PCR. (I) In situ hybridisation of Xenopus laevis embryos at the early gastrula stage confirms that inhibition of Smicl function causes the downregulation of Chordin. The embryo on the left was injected with coMO (80 ng; n=15) and the one on the right with XlMO (80 ng; n=15). (J) Injection of Chordin mRNA can partially rescue the phenotype caused by XlMO. Numbers indicate how many embryos out of 50 display this phenotype.
Image published in: Collart C et al. (2005)
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