Larger Image Fig. 8. Sia/Twn promote hhex transcription by inhibiting BMP and Vent. In situ hybridization of −6.0Kb hhex:gfp transgenic embryos injected as follows: Sia/Twn-MOs (20 ng each, 4-cell dorsal), Sia/Twn-MOs (20 ng each, 4-cell dorsal) + tBR mRNA (400 pg, 8-cell dorsal–vegetal), Vent1-MO + Vent2-MO (30 ng each; 4-cell ventral), Sia/Twn-MOs (20 ng each, 4-cell dorsal) + Vent1/2-MOs (30 ng each, 8-cell dorsal–vegetal), Chordin-MO + Noggin-MO (10 ng each, 4-cell dorsal) or sia mRNA (50 pg, 8-cell ventral–vegetal). Image published in: Rankin SA et al. (2011) Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue hhex.L hex, tHex, XHex X. laevis Throughout NF stage 10.5 endoderm endomesoderm anterior chrd.S chd, chordin, chrd.1, LOC108716939, X-chordin X. laevis Throughout NF stage 10.5 axial mesoderm involuted dorsal mesoderm ventx1.2.S vent-1, vent1, Xvent-1, Xvent1 X. laevis Throughout NF stage 10.5 involuted ventral mesoderm endoderm ventx2.2.L vent-2, vent2, Xom, xvent-2, xvent2 X. laevis Throughout NF stage 10.5 involuted ventral mesoderm endoderm ventral Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + chrd MO + nog MO + NF11 (in situ hybridization) fig.8 Expression Phenotype decreased amount chrd.1.L expression in dorsal endoderm decreased amount hhex.L expression in dorsal endoderm increased amount ventx1.2.L expression in animal cap mislocalised ventx1.2.L expression in animal cap increased amount ventx1.2.L expression in dorsal endoderm mislocalised ventx1.2.L expression in dorsal endoderm increased amount ventx2.2.L expression in animal cap mislocalised ventx2.2.L expression in animal cap increased amount ventx2.2.L expression in dorsal endoderm mislocalised ventx2.2.L expression in dorsal endoderm Xla Wt + sia1 + NF11 (in situ hybridization) fig.8 Expression Phenotype increased amount chrd.1.L expression in ventral endoderm mislocalised chrd.1.L expression in ventral endoderm mislocalised hhex.L expression in ventral endoderm increased amount hhex.L expression in ventral endoderm increased amount ventx1.2.L expression in animal cap mislocalised ventx1.2.L expression in animal cap decreased amount ventx1.2.L expression in ventral endoderm increased amount ventx2.2.L expression in animal cap mislocalised ventx2.2.L expression in animal cap decreased amount ventx2.2.L expression in ventral endoderm Xla Wt + sia1 MO + sia2 MO + NF11 (in situ hybridization) fig.8 Expression Phenotype decreased amount chrd.1.L expression in dorsal endoderm decreased amount hhex.L expression in dorsal endoderm increased amount ventx1.2.L expression in dorsal endoderm mislocalised ventx1.2.L expression in dorsal endoderm increased amount ventx2.2.L expression in dorsal endoderm mislocalised ventx2.2.L expression in dorsal endoderm Xla Wt + ventx1.1 MO + ventx2.1 MO + NF11 (in situ hybridization) fig.8 Expression Phenotype mislocalised chrd.1.L expression in ventral endoderm increased amount chrd.1.L expression in ventral endoderm mislocalised hhex.L expression in ventral endoderm increased amount hhex.L expression in ventral endoderm Xla Wt + dnbmpr1a + sia1 MO + sia2 MO + NF11 (in situ hybridization) fig.8 Expression Phenotype decreased amount chrd.1.L expression in dorsal endoderm decreased amount hhex.L expression in dorsal endoderm decreased amount ventx1.2.L expression in dorsal endoderm increased amount ventx1.2.L expression in ventral endoderm decreased amount ventx2.2.L expression in dorsal endoderm increased amount ventx2.2.L expression in ventral endoderm Permanent Image Page Printer Friendly View XB-IMG-48875