Fig. 4. Expression of wnt-3a and fgf-8 in regenerating limb buds. (A and D) Stage 52 limb buds. (B, C, E and F) Regenerating blastemas at 3 dpa. Right panels (C and F) show in situ hybridization on sectioned samples. Wnt-3a and fgf-8 are expressed in the inner layer of thickened apical epithelium of the blastema at 3 dpa. No specific hybridization signal was detected with an wnt-3a sense probe (C, inset). Arrowheads show amputation level (knee level). a, anterior; p, posterior; d, dorsal; v, ventral. Scale bars: 100 μm.
Image published in: Yokoyama H et al. (2007)
Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| wnt3a.S | wnt-3a, Xwnt-3a, xwnt3a | X. laevis | Throughout NF stage 52 | epithelium limb bud hindlimb bud distal hindlimb regenerating hindlimb blastema apical epithelial cap |
| fgf8.L | AIGF, FGF-8, fgf-8b, fgf8a, fgf8b, MGC79739, Xfgf-8, Xfgf8 | X. laevis | Throughout NF stage 52 | epithelium limb bud hindlimb bud anterior regenerating hindlimb apical epithelial cap blastema distal |
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