Fig. 6. Lower level reduction of FGF8a and FGF8b or strong reduction of FGF8a alone with XlMOF8, MOSDF8 and MOSAF8a prevents proper formation of posterior neural tissue at the early neurula stage.X. laevis embryos displayed dorsoanteriorly. Pink staining indicates the lineage tracer; embryos injected into one cell at the two- or four-cell stage. (A,E,I,M) Control MO (40 ng); (B,F,J,N) MOF8 20 ng; (C,G,K,O) MOSDF8 43 ng; (D,H,L,P) MOSAF8a 60 ng. (A-D) XlMOF8 (44/45), MOSDF8 (18/19) and MOSAF8a (38/39) cause a mispatterning of sox2 expression. (E-H) otx2 expression is expanded toward the posterior after XlMOF8 (23/25), MOSDF8 (20/20) and MOSAF8a (26/29) injection, while the posterior neural gene dbx is absent (MOF8, 25/25; MOSDF8, 20/20; MOSAF8a, 35/38). (I-L) en2 expression is diminished and sometimes completely absent on the injected side: XlMOF8 (13/13), MOSDF8 (17/17) and MOSAF8a (32/33). (M-P) both the spinal cord domain (hoxB9) and the hindbrain domain (krox20) is strongly reduced and shifted toward the posterior of the embryo on the XlMOF8 (42/42), MOSDF8 (20/20) and MOSAF8a (40/40) injected side of the embryo. Effects on the uninjected side are present but much weaker. (Q-S) Neural tube stage 20 embryos treated as indicated; (T-V) FGF8a mRNA (50 pg) rescued the reduction of hoxB9 caused by XlMOF8 (32/44), MOSAF8a (19/35) and MOSDF8 (19/20).
Image published in: Fletcher RB et al. (2006)
Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| sox2.S | anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 | X. laevis | Throughout NF stage 20 | neural tube |
| otx2.L | otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 | X. laevis | Throughout NF stage 20 | forebrain |
| dbx1.L | dbx, Xdbx | X. laevis | Throughout NF stage 20 | posterior neural tube |
| en2.L | En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 | X. laevis | Throughout NF stage 20 | midbrain |
| myod1.L | mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD | X. laevis | Throughout NF stage 20 | paraxial mesoderm presomitic mesoderm |
| egr2.L | EGR-2, Krox-20, krox20, XKr20, XKrox-20 | X. laevis | Throughout NF stage 20 | rhombomere R3 rhombomere R5 |
| hoxc9-like.L | X. laevis | Throughout NF stage 20 | posterior neural tube |
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