Fig. 3. Interference with ribosome biogenesis phenocopies PPan knockdown. (A) Xenopus 28S, 18S and 5.8S rRNAs are transcribed as a common rRNA precursor separated by the internal transcribed spacers 1 and 2 (ITS1 and ITS2). The binding site of ITS2 MO is indicated. ETS, external transcribed spacer. (B) Both PPan MO and ITS2 MO injection resulted in reduced 5.8S rRNA levels in anterior neural tissue at stage 23. Ten individual explants per MO were analyzed by RT-PCR. The agarose gel shows representative examples for reduced (ITS2 MO) and strongly reduced (PPan MO) signals. qRT-PCR analyses are also given (right). The data are presented as relative units normalized to Control MO-injected samples. (C,D) ITS2 MO injection into one animal-dorsal blastomere at the 8-cell stage results in severe eye (C) and craniofacial cartilage (D) defects in a dose-dependent manner (arrows). Quantitative evaluations are given beneath. n, number of independent experiments; N, total number of embryos examined. Error bars indicate s.e.m.
Image published in: Bugner V et al. (2011)
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