Fig. 7. Loss of ionocytes affects the development of ciliated and goblet cells. (A,B) SEM and (C,D) TEM images of stage 27 tadpole epidermis of control and foxi1e-ATG-MO-injected embryos, as indicated. (A) Knockdown of foxi1e causes loss of cells with apical pits (ionocytes) but ciliated cells are still present. Scale bars: 25 μm. (B) High-magnification images reveal that ciliated cells in foxi1e knockdown embryos possess fewer cilia than controls. Scale bars: 10 μm. (C) TEM images show that, in the control, basal bodies are located beneath the apical membrane (arrowheads), whereas, in the foxi1e morphant embryos, numerous basal bodies are abnormally located deep in the cytoplasm (arrowheads). Scale bars: 1 μm. (D) Goblet cells develop an increased number of cellular protrusions, resembling microvilli, in the foxi1e morphant embryos (arrowheads) compared with goblet cells of control embryos. Scale bars: 1 μm (control); 500 nm (foxi1e ATG MO).
Image published in: Dubaissi E and Papalopulu N (2011)
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