Figure 2. Recovery of the A7 CAR:Cre strain. Frozen sperm from the A7 male was microinjected into eggs derived from the C5 reporter strain containing the LCMV:ECFP(loxP)(FRT)EYFP transgene. A: Stage 42 tadpole showing blue fluorescence of the ECFP marker gene. B: The same tadpole in the yellow filter set shows yellow fluorescence in tail myotomes. C: A Cre-positive tadpole with the activated EYFP reporter gene in myofibrils of the jaws and the abdominal wall. Stage 52, ventral view. D: Tail-tip polymerase chain reaction analysis for the presence of Cre in tadpoles with or without activation of the reporter gene in muscle cells. Tadpoles from the same crossing without the reporter gene were also analyzed. Scale bars = 1 mm.Download figure to PowerPoint
Image published in: Waldner C et al. (2006)
Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons.
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