Fig. 2. Retinoid depletion causes radical molecular truncation of the posterior hindbrain by the end of gastrulation. (Top panel) Whole- mount in situ hybridizations (wISH) on st. 20 Xenopus laevis embryos (A- F). The upper row shows non-treated embryos (indicated by control) and the bottom embryo is treated with 10-6 M AGN (indicated by AGN). All views are dorsal and anterior at the top. (A) Hoxb-1, arrowhead indicates hindbrain expression; (B) Hoxd-3, ar- rowhead indicates hindbrain expression; (C) En2, Krox-20 and Hoxb-4, top arrowhead indicates En stripe, bottom arrowheads indicate Krox-20 stripes and bar indicates Hoxb-4 stripe; (D) Hoxa-5, arrowhead indicates hindbrain expression and bar indicates spinal cord expression; (E) Krox-20 and Hoxc- 6, arrowhead indicates posterior Krox-20 stripe, bar indicates Hoxc-6 expression; (F) Otx-2 and Xcad3, bar indicates gap between Otx-2 (anterior) and Xcad3 (posterior) expression patterns; (G) XlPOU2, arrowhead indicates hindbrain expression and bar indicates spinal cord expression. (Bottom panel) Whole-mount in situ hybridizations on st. 13 Xenopus laevis embryos (H-M). The upper row shows non-treated embryos (indicated by control) and the right embryo is treated with 10-6 M AGN (indicated by AGN). All views are dorsal and anterior at the top. (H) Hoxd-1; (I) Hoxa-1; (J) Hoxb-1; (K) Hoxd-3; (L) Krox-20 (anterior stripes) and Hoxb-4; (M) Krox-20 (anterior stripes) and Hoxc-6. Arrows in pictures (L,M) localise sparse cells representing the posterior stripe of Krox-20.
Image published in: Lloret-Vilaspasa F et al. (2010)
Copyright © 2010. Reproduced with permission of the Publisher, University of the Basque Country Press.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| hoxb1.L | hox-2.9, hoxb-1, XHox-b1 | X. laevis | Throughout NF stage 20 | hindbrain mesoderm lateral plate mesoderm neuroectoderm |
| hoxd3.L | hox-d3 | X. laevis | Throughout NF stage 20 | hindbrain rhombomere R5 neural tube neuroectoderm |
| hoxa5.S | hox-a5, hox1.3, hox1c | X. laevis | Throughout NF stage 20 | hindbrain rhombomere R5 neural tube neuroectoderm |
| en2.L | En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 | X. laevis | Throughout NF stage 20 | hindbrain presumptive midbrain-hindbrain boundary neuroectoderm |
| egr2.L | EGR-2, Krox-20, krox20, XKr20, XKrox-20 | X. laevis | Throughout NF stage 20 | hindbrain rhombomere R3 rhombomere R5 neural tube cranial neural crest neuroectoderm |
| hoxb4.S | LOC494794, XHox-1a, XHox-b4, xHox1a | X. laevis | Throughout NF stage 20 | neural tube neuroectoderm |
| hoxa5.S | hox-a5, hox1.3, hox1c | X. laevis | Throughout NF stage 20 | hindbrain neural tube neuroectoderm |
| hoxc6.S | ACI, xeb1, XlHbox1 | X. laevis | Throughout NF stage 20 | neural tube neuroectoderm |
| otx2.S | otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 | X. laevis | Throughout NF stage 20 | eye primordium neuroectoderm |
| cdx4.L | cad3, MGC130769, MGC68909, Xcad-3, Xcad3, Xtcad3 | X. laevis | Throughout NF stage 20 | neural tube neuroectoderm |
| pou3f4.L | brn4, POU 2, pou2, XlPOU 2, XlPOU-2, XlPOU2 | X. laevis | Throughout NF stage 20 | hindbrain lateral plate mesoderm neuroectoderm |
| hoxd1.S | MGC131028, Xhox.lab, xhox.lab1, xhoxd1, xlab, Xlab-1 | X. laevis | Throughout NF stage 13 | involuted dorsal mesoderm neuroectoderm |
| hoxa1.L | bsas, hox1f, hoxa1-a, hoxa1-b, Xhox.lab, xhox.lab2, xhoxa1 | X. laevis | Throughout NF stage 13 | mesoderm |
| hoxb1.L | hox-2.9, hoxb-1, XHox-b1 | X. laevis | Throughout NF stage 13 | mesoderm notochord neuroectoderm |
| hoxb1.L | hox-2.9, hoxb-1, XHox-b1 | X. laevis | Throughout NF stage 13 | dorsal mesoderm notochord neuroectoderm |
| hoxd3.L | hox-d3 | X. laevis | Throughout NF stage 13 | hindbrain mesoderm neuroectoderm |
| hoxb4.S | LOC494794, XHox-1a, XHox-b4, xHox1a | X. laevis | Throughout NF stage 13 | mesoderm notochord neuroectoderm |
| hoxc6.S | ACI, xeb1, XlHbox1 | X. laevis | Throughout NF stage 13 | mesoderm |
| egr2.L | EGR-2, Krox-20, krox20, XKr20, XKrox-20 | X. laevis | Throughout NF stage 13 | rhombomere R3 rhombomere R5 neuroectoderm |
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