Fig. 2. α-Tubulin-mGFP transgene marks CCPs. (A-C) mGFP fluorescent images of live embryos that are transgenic forα -tubulin-mGFP at the indicated stages. In A, the boundary between the neural plate (NP) and non-neural ectoderm is denoted with a broken line. (D,E) α-tubulin-mGFP transgenic embryos were fixed at stage 14 (early neurulae) and probed using whole-mount in situ hybridization to detect either mGFP RNA (D) or α-tubulin RNA (E). Transverse sections of stained embryos on the ventral side with the inner (IL) and outer layer (OL) of ectoderm labeled. (F)α -tubulin-mGFP transgenic embryos were fixed at stage 28 and stained with an antibody directed against cilia (red fluorescence). The mGFP (green) and antibody staining (red) overlap (yellow).
Image published in: Stubbs JL et al. (2006)
Copyright © 2006. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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