Fig. 2. Lef1 depletion results in impaired development of different tissues. Non-injected control X. tropicalis embryo, stage 38 (A). Lef1 morpholino oligo injected embryos (B) are short and lack a tail with a dorsal and ventral fin. The eyes are small and pigmentation is absent. Co-injection of mRNA encoding human LEF1 rescues the lef1 MO effects (C). Broken lines in (A-C) indicate level of sections shown in (D-F). Transverse section of non-injected control embryo (D). Lef1 MO injected embryos (E) lack the dorsal longitudinal anastomosing vessel (dlav), the posterior cardinal veins (pcv), the pronephric ducts (pd), the hypochord (hc) and the dorsal aorta. The somites and notochord (N) are poorly differentiated. The spinal cord (sc) lacks nerve fibers and the notochord (N) is smaller in diameter. Embryo co-injected with LEF1 RNA (F) shows rescue of the structures affected by lef1 depletion. 3D reconstructions of transverse sections (G-H of embryos shown in (A,B). Noninjected control embryo (G,G and embryo injected with lef1 MO (H,H. Reconstructions were aligned relative to the otic vesicle.
Image published in: Roel G et al. (2009)
Copyright © 2009. Reproduced with permission of the Publisher, University of the Basque Country Press.
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