Figure 4. Overexpression of PIAS disrupts early mesodermal formation. In situ hybridization was performed to assess mesodermal formation in embryos injected with PIAS RNAs. While all four PIAS genes inhibited expression of Chordin, XBra, and MyoD, they displayed different efficiency in doing so, with PIAS3 having weak XBra inhibitory activities. Unlike PIAS1, 2, and 4, PIAS3 did not block expression of Wnt8 efficiently. The doses of the RNAs used in this experiment were: 1 ng PIAS1, 0.5 ng PIAS2, 2 ng PIAS3, and 1 ng PIAS4.Download figure to PowerPoint
Image published in: Burn B et al. (2011)
Copyright © 2011. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| tbxt.S | bra, brachyury, ntl, t, t-a, t-b, X-bra, Xbra, Xbrachyury | X. laevis | Throughout NF stage 10.5 | involuting marginal zone mesoderm marginal zone |
| chrd.S | chd, chordin, chrd.1, LOC108716939, X-chordin | X. laevis | Throughout NF stage 10.5 | marginal zone |
| myod1.S | mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD | X. laevis | Throughout NF stage 10.5 | dorso-lateral marginal zone mesoderm |
| wnt8a.L | wnt-8, wnt8, Xwnt-8, Xwnt8 | X. laevis | Throughout NF stage 10.5 | ventral marginal zone ventro-lateral marginal zone |
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