Fig. 5. XSufu is important for cement gland, neural crest, neuronal and paraxial mesoderm development. Embryos were injected together with nlacZ mRNA as tracer animally into one blastomere at the 2- or 4-cell stage. (A,A′,B,B′) Whole-mount in situ hybridization of early neurula embryos in anterior view. XSufu mRNA and XSufu-MO lead to a reduction of the cement gland marker XAG while a non-specific control-MO has no effect. (C–F,C′–F′,D′,F′) Late gastrulae in dorsal and tail bud embryo in lateral view. XSufu mRNA and XSufu-MO cause a reduction of the neural crest markers Slug and Snail. Co-injection of XSufu-MO and XSufu* mRNA reverts to normal Slug and Snail expression. (G,G′,H–H′) Early neurulae in dorsal view. XSufu mRNA causes slight expansion of the neuronal marker N-tubulin. XSufu-MO reduces N-tubulin expression. Normal N-tubulin expression is seen after co-injection of XSufu-MO with XSufu* mRNA. (I–I′) Tail bud embryos in dorsal view. XSufu-MO inhibits formation of MyoD-positive segmented somites (see also magnification in inset). Indicated effects were observed in: A′, 9/16; B, 40/45; B′, 7/7; C′, 70/70; D, 65/68; D′, 14/15; D′, 62/64; E′, 62/65; F, 13/13; F′, 19/19; F′, 30/33; G′, 66/81; H, 102/105; H′, 78/84; H′, 31/39; I, 15/20; I′, 17/17.
Image published in: Min TH et al. (2011)
Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| snai2.L | slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 | X. laevis | Throughout NF stage 14 | neural crest neuroectoderm |
| snai1.S | LOC108702592, sna, snail, snail1, xSna, Xsnail | X. laevis | Sometime during NF stage 18 to NF stage 26 | neural crest cranial neural crest mandibular crest hyoid crest anterior branchial crest posterior branchial crest |
| ag1.L | ag, ag1-a, ag1-b, XAG, xag-1, xag1, Xag2 | X. laevis | Throughout NF stage 17 | cement gland primordium |
| tubb2b.S | N-Tub, n-tubulin, NBT, neural beta-tubulin, NST, ntubulin, Xn-tubulin | X. laevis | Throughout NF stage 14 | neural plate neuron neural groove neuroectoderm |
| myod1.S | mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD | X. laevis | Throughout NF stage 24 | mesoderm paraxial mesoderm somite presomitic mesoderm |
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