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Fig. 2. Incorporation of Xenopus-compatible elements into pTransgenesis. (A) Schematic of recombination with a Xenopus-compatible p4 vector containing a single I-SceI site, Tol2 elements, and SAR-CH4 sequences, p3 Katushka RFP and Xenopus-compatible p1 and p2 constructions. (B-K) Transgenic tadpoles from the resulting recombinations are shown. Individual p1, p2, p3 constructions and expression domains are written on each panel in white. Images in E′ and G′ are magnified images of the boxed regions in E and G, respectively. Image in F′ shows the induction of RFP from the heat-shocked tadpole in F.

Image published in: Love NR et al. (2011)

Copyright © 2011. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

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