Fig. 4. Dorsoanteriorly directed force on the surface of the non-neural ectoderm. (A-C) Laser ablation experiment in non-neural ectoderm. Fluorescent images of a memGFP-injected embryo were taken just before (A) and immediately after (1.5 seconds; B) the incision. These images were merged after image processing, and the displacement of cell vertices between the two time points was measured (C). (D,E) Sectional views of an embryo fixed immediately after incision. A gap was made on the surface of the embryo without affecting the internal morphologies (D). White boxed region in D is magnified in E. Asterisk indicates deep cells under the ablation site. (F-H) Laser ablation along the AP axis. Lateral non-neural ectoderm of neurula embryos was ablated along the AP axis (F, red line). Distance (G) and mean angle (H) of the displacement were measured on the dorsal and ventral sides of the ablation line, respectively, in early (stage 13) to mid- (stage 16) neurula embryos. n=27, 23, 16 and 21 (from stage 13 to stage 16); data are mean±s.e.m.; *P<0.05, t-test. (I-K) Laser ablation along the DV axis. The lateral side of the neurula was ablated along the DV axis (I, red line). The distance (J) and mean angle (K) were measured on the anterior and posterior side. n=6, 5, 7 and 8 (from stage 13 to stage 16); data are mean±s.e.m.; *P<0.05, t-test. Scale bars: 100 μm in D; 20 μm in A,E.
Image published in: Morita H et al. (2012)
Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
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