Figure 7. Reciprocal presumptive lens ectoderm (PLE) transplants. Embryos were injected unilaterally at the two-cell stage with either morpholino or rhodamine green dextran, and PLEs were transplanted between injected animals at stage 14. The resulting stage 35 larvae are shown. For the panels in the first three columns, anterior is to the right and dorsal is up. Arrows indicate the locations of eyes. For the panels in the fourth column, dorsal is to the right. Each row shows, left to right, one embryo as whole-mount in transmitted light, red morpholino fluorescence, and green dextran fluorescence, and the affected eye in section, respectively. A–D: Rhodamine green dextran host with a red lissamine Xenopus MADM-like (xMADML) morpholino (MO) PLE transplant. E–H: Red lissamine xMADML-MO host with rhodamine green dextran PLE transplant. I–L: Rhodamine green dextran host with a red lissamine control morpholino (CTL-MO) PLE transplant. M–P: Red lissamine CTL-MO host with a rhodamine green dextran PLE transplant. ce, corneal epithelium; le, lens; nr, neural retina. Scale bars = 400 μm in the first three columns, 100 μm in the fourth column.Download figure to PowerPoint
Image published in: Elkins MB and Henry JJ (2006)
Copyright © 2006. Image reproduced with permission of the Publisher, John Wiley & Sons.
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