Figure 2. Chromosomal location of Tol2 transposon insertions in the Xenopus tropicalis genome. (a) Schematic representation of Xenopus tropicalis chromosomes and the relative location of the Tol2 integration sites (not to scale). Chromosomal assignments were predicted using the scaffold identity revealed by comparison of the transposon flanking sequence with the Xenopus tropicalis genome sequence (JGI v4.1 assembly). The position of the Tol2 integration on each chromosome was estimated from the map position of the corresponding scaffold. The parental integration sites (10M and 12M (hbr)) used for somatic remobilization are depicted by the thick lines and are labeled on the left side of the chromosome. Examples of remobilized somatic transposition events listed in Figure 1b are labelled on the right hand side of the chromosome. Mapping the integration sites provided evidence for both 'local hopping' and random re-integration throughout the genome. (b) Fluorescent in situ hybridization (FISH) analysis of the 10M line confirms the location of the Tol2XIG transposon on the long arm of chromosome 8 (linkage group 5). Metaphase spreads prepared from heterozygous 10M tadpoles were hybridized with a digoxigenin-labeled GFP probe and counterstained with DAPI.
Image published in: Yergeau DA et al. (2010)
Copyright ©2010 Yergeau et al; licensee BioMed Central Ltd. This image is reproduced with permission of the journal and the copyright holder. This is an open-access article distributed under the terms of the Creative Commons Attribution license
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