Fig. 6. The extent of the vascular plexus is related to the size of the hand1 domain. To more directly assess the relationship between the forming vasculature and hand1 expression double in situ hybridizations were done with hand1 being visualized in light blue and etv2, marking the forming vasculature, in purple. The forming vascular plexus (emphasized in the lower panels of each example) along the side of the embryo does not extend to the end of the embryo in control (DMSO, ATP) embryos nor does the hand1 staining. When the extent of the vascular plexus is shifted towards the anterior end by treatment with RAA (A and D) this is reflected in the changes to the hand1 expression domain. When the vascular plexus is shifted towards the end of the embryo by addition of RA (C and F) or SU5402 (H and J) this is again reflected in the changes to the extent of hand1 expression domain along the anteriorposterior axis. Note that the close correspondence appears to be lost in the dorsal ventral axis in the SU5402-treated embryos (H and J).
Image published in: Deimling SJ and Drysdale TA (2011)
Copyright © 2011. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| hand1.L | eHand, hand-1 | X. laevis | Throughout NF stage 28 | lateral plate mesoderm |
| etv2.L | er71, ets1, ets2-like, Etsrp, etsrp71 | X. laevis | Throughout NF stage 28 | cardinal vein intersomitic vessel aortic arch |
Image source: Published
| Experiment + Assay | Source | Phenotypes and Disease | ||||
|---|---|---|---|---|---|---|
| Xla Wt + retinol + NF32 (in situ hybridization) | Fig. 6 CF |
| ||||
| Xla Wt + su5402 (@12.5) + NF32 (in situ hybridization) | Fig. 6 HJ |
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