Fig. S3. Co-transgenic reporter analysis of cer regulatory regions and responsiveness of cer to VegT. (A) Co-transgenesis for searching cer regulatory regions. The PstI restriction site of the 5′ end of the cer EGFP reporter cassette −62Xt_cer and the 3′ end of PCR fragments (3′ primers have a PstI site) were digested by PstI. Sperm nuclei incubated with these fragments were injected into unfertilized eggs, and normal cleaved eggs were incubated until the gastrula stage. Fixed embryos were bisected along the dorsal midline and whole-mount in situ hybridization was performed for EGFP mRNA. Expression levels were divided into two groups as indicated. (B) Induction of the endogenous cer expression by VegT in the animal cap region. vegt mRNA (100 pg/embryo) was injected into the animal pole region of one blastomere at the two-cell stage. An arrowhead indicates the injected side. (C) Responsiveness of cer reporters to VegT. The reporter construct −229Xt_cer/EGFP with or without vegt mRNA (100 pg/embryo) was injected into the animal pole region at the two-cell stage. Expression of the cer (B) or reporter (C) gene was examined by whole-mount in situ hybridization with antisense probes as indicated.
Image published in: Sudou N et al. (2012)
Copyright © 2012. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| cer1.S | cer, cer-1, Cerberus, dand4, LOC108713989, tCerberus, xcer, xcer-1, xcer1 | X. laevis | Throughout NF stage 11 | endoderm endomesoderm |
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