Fig. 3. Expression of xSP-C and xSP-B at different developmental time points. RT-PCR was performed on cDNA from various developmental time points in order to determine when xSP-C and xSP-B genes were expressed in the developing embryo. The following cDNA was used in each lane: (1) water, (2) adult lung with no reverse transcriptase added to the RT reaction, (3) blank lane, (4) stage 2, (5) stage 6.5, (6) stage 10, (7) stage 15, (8) stage 21, (9) stage 24, (10) stage 27, (11) stage 31, (12) stage 33/34, (13) stage 35/35, (14) stage 41, (15) stage 43, (16) adult lung. xSP-C and ODC expression was detected after 35 cycles of PCR and xSP-B was detected after 30 cylces.
Image published in: Hyatt BA et al. (2007)
Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V.
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