Fig. 4. Zic3 inhibits secondary axis formation induced by ectopic β-catenin. Typical shapes of the tailbud stage embryos (stage 33, dorsal view) subjected to the second axis formation assay are shown. Percentages given below indicate the embryos with secondary axis among 43–50 embryos for each experimental group. Flag-tagged β-catenin RNA (200 pg) injected into the ventral marginal zone at the 4-cell stage frequently induced the secondary axis structure (B; β-catenin, 78%). The secondary head structure was rarely observed after co-injection of Flag-tagged β-catenin RNA (200 pg) and Zic3 RNA (50 pg) (C; β-catenin + Zic3, 4%). The suppression of the secondary axis effect by Zic3 RNA was abolished by Zic3MO (10 ng) (E; β-catenin + Zic3 + Zic3MO, 70%). There were no embryos with ectopic heads in Zic3MO-injected (D; Zic3MO, 0%), Zic3 RNA-injected (F; Zic3, 0%), or control embryos without injection (A; uninjected, 0%).
Image published in: Fujimi TJ et al. (2012)
Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V.
Permanent Image Page
Printer Friendly View
XB-IMG-77656