Supplemental figure 2. Temporal expression patterns of Zic genes The temporal expression patterns of Zic genes were determined by RT-PCR analysis. Staging was done according to Nieukoop and Farber (1967) (N.F. stage). Zic1 showed strong expression in early stages: Zic2 was maternally expressed, and Zic1 and Zic3 expression began at the gastrula stage. The primers for Xenopus Zic4 detection were F: 5′-TCTCAGTGACTACTGCCA-3′ and R: 5′-GACATGAAGAATTCACCGTC-3′. Other primers for Xenopus Zic genes were as previously described (Nakata et al., 2000). e, unfertilized egg.
Image published in: Fujimi TJ et al. (2012)
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