Fig. 11. mHoxb1 fails to rescue somitogenesis. Non-injected control (A) and embryos injected in the LHS with mHoxb1 mRNA (B: 100%, n=27), PG1 MOs (C: 81%, n=32) or a combination of PG1 MOs and mHoxb1 (D: 91% rescued, n=23), were analysed for the expression of MyoD (somites), Krox-20 (rhombomeres 3 and 5) and engrailed-2 (midbrain–hindbrain boundary) at early tailbud stage. Embryos are shown from a lateral view with anterior side to the left. The co-injection of mHoxb1 fails to rescue the somite, as shown by the continuous MyoD expression. Krox-20 expression shows that both PG1 MO and mHoxb1 overexpression is effective.
Image published in: Peres JN et al. (2006)
Copyright © 2006. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| egr2.L | EGR-2, Krox-20, krox20, XKr20, XKrox-20 | X. laevis | Throughout NF stage 26 | rhombomere R3 rhombomere R5 cranial neural crest |
| en2.L | En-2, en2-a, en2-b, eng2, engrailed 2, engrailed homolog 2, engrailed-2, maben, xen2 | X. laevis | Throughout NF stage 26 | midbrain-hindbrain boundary |
| myod1.S | mf25, MyoD, myod1-a, myod1-b, myogenic factor 3, XMyoD, XtmyoD | X. laevis | Throughout NF stage 26 | somite muscle epaxial muscle |
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