Figure 7. Tiki1 Functions in Both Wnt-Producing and Wnt-Responding Cells(A) Tiki1 acts in Xwnt8-producing cells. Tiki1 mRNA (100 pg) coinjected with Xwnt8 mRNA (20 pg) inhibited Xwnt8-induced S01234-luciferase in neighboring cells. LDLR: a control.(B) Tiki1 acts in Xwnt8-responding cells. Tiki1 mRNA coinjected with S01234-luciferase inhibited the reporter induced by Xwnt8 from neighboring cells that received Xwnt8 mRNA.(C) The endogenous Tiki1 acts in Wnt-producing cells. Tiki1MO (20 ng) coinjected with CS2+Xwnt8 DNA (40 pg) in a single dorsal blastomere enhanced Xwnt8-induced S01234-luciferase in neighboring cells. ConMO: control MO.(D) The endogenous Tiki1 acts in Wnt-responding cells. Tiki1MO coinjected with S01234-luciferase enhanced the reporter induced by Xwnt8 from neighboring cells that received CS2+Xwnt8 DNA.(E–N) Tiki1 reduces nuclear β-catenin levels by acting in Wnt-responding cells. Tiki1 or LDLR mRNA (200 pg) (FLD+, green) and Xwnt8 mRNA (20 pg) (RFP+, red) were injected into neighboring blastomeres. Stage 9 animal cap cells were imaged with an anti-β-catenin antibody (blue). Examples of Xwnt8-expressing cells (arrows) and Tiki1- or LDLR-expressing cells (arrowheads) are highlighted. Only cells within the distance of five cell bodies from Xwnt8-expressing cells were counted. No nuclear β-catenin-positive cells were found in areas free of Xwnt8-expressing cells (not shown). All cells were positive for plasma membrane-associated β-catenin. Statistical data were derived from three independent experiments (F). ∗∗p < 0.001.All error bars represent SD in triplicates. See also Figures S7.
Image published in: Zhang X et al. (2012)
Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V.
| Experiment + Assay | Source | Phenotypes and Disease | ||
|---|---|---|---|---|
| Xtr Wt + trabd2b + wnt8a + NF16 (in situ hybridization) | fig.7.k-n |
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