Larger Image Fig. S2. XSeb4R gain- and loss-of-function in the embryos. Pigmented and albino embryos were unilaterally injecetd with XSeb4R capped-RNA (200 pg/blastomere) or XSeb4R-MO (20 ng/blastomere), using LacZ mRNA as a tracer (200 pg/blastomere). Embryos were cultured and fixed at neurula stage 18 and at tadpole stage. X-gal staining were performed (blue color) and the albino embryos were analyzed by WMISH. Note differential distribution of the blue color, recapitulating the sites of XSeb4R overexpression (A) or depletion (B). Neural crest (NC) formation was investigated targeting Slug expression (B). XSeb4R-MO revealed no significant effect on the intensity of Slug expression but rather a marked clustering of signal that reflects NC cells migration inhibition (100%, n = 32). Image published in: Bentaya S et al. (2012) Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V. Gene Synonyms Species Stage(s) Tissue snai2.L slug, snai2-a, snai2-b, Snail2, xSlu, xslug, XSnail2 X. laevis Throughout NF stage 18 neural crest migratory neural crest cell premigratory neural crest cell Image source: Published Experiment + Assay Source Phenotypes and Disease Xla Wt + rbm38 + NF37/38 (morphology) fig.S2.a Anatomical Phenotype abnormal bending of anterior-posterior axis abnormal head morphology absent eye increased pigmentation in the trunk increased size of the ventral trunk Xla Wt + rbm38 MO + NF18 (morphology) fig.S2.b Anatomical Phenotype abnormal cell migration in neural crest Expression Phenotype mislocalised snai2.L expression in migratory neural crest cell Permanent Image Page Printer Friendly View XB-IMG-78848