Figure 5. The IP/Total ratio and Specificity Index (SI) provide independent measures to identify genes enriched above background levels in different cell types. A,B: Data for each of three biological replicates are plotted as the ratio of the absolute expression level for the IP for Tg(isl2b:EGFP-Rpl10a) (A) and Tg(rho:EGFP-Rpl10a) (B) TRAPed mRNAs over the absolute expression level for RNAs purified from total unbound fraction from a reference wildtype sample (Total) for each gene. Standard error of the mean (error bars) was calculated based on the standard deviation for a ratio as described in the Experimental Procedures section. C: Scatter plot of the SI for all cell types plotted against individual genes shows a clustering of cell type–specific enrichment for RGCs (red highlighted cluster) and rod phototoreceptor cells (blue highlighted cluster). Genotypes on graph are as indicated isl2b = Tg(isl2b:EGFP-rpl10a); rho = Tg(rho:EGFP-rpl10a); fabp7 = Tg(fabp7:EGFP-rpl10a). P values are significant at an SI less than 4 (dashed line, P < 0.1).Download figure to PowerPoint
Image published in: Watson FL et al. (2012)
Copyright © 2012. Image reproduced with permission of the Publisher, John Wiley & Sons.
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