Fig. 1. XNr1/Cripto and caAlk4 misexpression induce ectopic expression of cardiac genes Nkx2.5, Tbx5, TnIc and αMHC cell non-autonomously. (A) One ventral blastomere of 16–32 cell stage embryos were injected with mRNAs encoding XNr1 and Cripto or caAlk4 along with AD546 (red) as a lineage label. The normally non-cardiogenic VMZ mesendoderm was explanted at the onset of gastrulation (stage 10.25–10.5), cultured until cardiac tissue expressed Nkx2.5 and Tbx5 (stage 23–25) or Tnic and αMHC (stage 30), then stained for gene expression by in situ hybridization (blue). (B–G) Examples of cell non-autonomous gene induction on histological section. Injection of XNr1 and Cripto or caAlk4 mRNAs with AD 546 induced Nkx2.5 (B, F), Tbx5 (D) TnIc (E, G) and αMHC (C). Images represent a photo merging of brightfield and fluorescent images. Note that cardiac marker positive cells (blue) were localized at a distance from lineage-labeled cells (red). Scale bar in panels B, D–G represents 40 μM, scale bar in panel C represents 20 μM.
Image published in: Foley AC et al. (2007)
Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V.
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