Fig. 6 – WSTF knockdown embryos display increased apoptosis of specific stages of development. A: TUNEL assay with a representative control embryo at tailbud stage showing no detectable levels of apoptosis (Stage 33; lateral view, anterior at left). B: A representative tailbud stage embryo injected with WSTF morpholino at the one-cell stage displaying a spatial increase in apoptosis in the hindbrain, olfactory placode and lateral anterior tissues. C: Side-by-side dorsal view of control embryo (left) with WSTF knockdown embryo (right) at tailbud stage, showing an increase in apoptosis in the hindbrain of the WSTF knockdown embryo compared to control (Stage 33; dorsal view, anterior at top). D: Transverse section of WSTF knockdown embryo (at a plane indicated by the dashed line in B) displays punctate staining in the peripheral tissues, lateral and ventral from the hindbrain, beneath the overlying epidermal ectoderm (Stage 33; sectional view, anterior at top). STD: standard control morpholino; MO: WSTF morpholino; nt: neural tube; nc: notochord. Scale bars: 0.5 mm (A and B), 0.1 mm (C and D).
Image published in: Barnett C et al. (2012)
Copyright © 2012. Image reproduced with permission of the Publisher, Elsevier B. V.
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